We do not capture any email address. THE mating-type (MT) locus of the haploid green alga Chlamydomonas reinhardtii, located 30 cM from the centromere of linkage group (chromosome) VI, is involved in generating mating-type plus or minus gametic phenotypes in response to nitrogen starvation (G oodenough et al. 201410 chlamydomonas.png 400 × 375; 20 KB. Nuclear fraction was prepared from cell wall–mutant CC4350+.

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, The European Molecular Biology Laboratory, State Secretariat for Education, Research and Innovation. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. 1995).The mt + and mt − versions of this locus segregate 2:2 at meiosis, but early genetic analysis documented … 95 (S ager and Z alokar 1958).The fn68 mutant was used to show that a rhodopsin photoreceptor controls the phototactic response in Chlamydomonas and that retinal, a derivative of carotenoids, … For in-gel hybridization analysis, gDNA was digested by the cocktail of enzymes following the same procedure, with some samples being pretreated with ExoT (50 units for 2 h at 25°C; New England Biolabs), which degrades single-stranded 3′ DNA extension and generates blunt ends, similarly to ExoI. The common laboratory strains are derived from a soil isolate, therefore, the natural environment of Chlamydomonas is close to that of land plants. The pellet was washed with 70% ethanol, dried, and resuspended in 25 μl H2O. Published 3 June 2019. One of the many striking features of Chlamydomonas is that it contains ion channels (channelrhodopsins) that are directly activated by light. The single celled organism has one chloroplast and moves via an anterior … After scanning, the membrane was stripped and reprobed using 18S-derived probe. Chlamydomonas reinhardtii ... Upload media Wikipedia Wikispecies: Instance of: taxon, model organism: Common name; For primer extension by the Φ29 polymerase, we used the C. reinhardtii–specific PETRA-T oligonucleotide 5′-CTCTAGACTGTGAGACTTGGACTACCCTAAAACCCT-3′ (Table S1). J Fulneček: formal analysis, investigation, methodology, and writing—review and editing. Cancer and telomeres: An ALTernative to telomerase, Interstitial telomere-like repeats in the Arabidopsis thaliana genome, A genome-wide screen for essential yeast genes that affect telomere length maintenance, Role of oxidative stress in telomere length regulation and replicative senescence, Development and characterization of genome-wide single nucleotide polymorphism markers in the green alga Chlamydomonas reinhardtii, Genetic control of chromosome length in yeast, Germline replications and somatic mutation accumulation are independent of vegetative life span in Arabidopsis, Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: Beginning to end, Saccharomyces telomeres assume a non-nucleosomal chromatin structure, Telomerase mechanism of telomere synthesis, Telomeres: Beginning to understand the end, Ku suppresses formation of telomeric circles and alternative telomere lengthening in Arabidopsis, Telomere length regulation in mice is linked to a novel chromosome locus, https://phytozome.jgi.doe.gov/pz/#!info?alias=Org_Creinhardtii, https://creativecommons.org/licenses/by/4.0/, Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants. In brief, the membrane was prehybridized at 42°C in Rapid-hyb buffer for 1 h, then the radioactive probe (20 pmol) was added, and the incubation was continued for 1 h. The membrane was washed consecutively with 5× SSC, 0.5% SDS (42°C for 10 min); 5× SSC, 0.1% SDS (42°C for 20 min); and 1× SSC, 0.1% SDS (25°C for 30 min). The green micro-alga Chlamydomonas reinhardtii is an elegant model organism to study all aspects of oxygenic photosynthesis. 1 ml of nuclei isolated from C. reinhardtii strain CC4350+ cw15 mt+ in buffer B was thawed on ice. Chlorophyll (Chl) and heme are major tetrapyrroles that play an essential role in energy metabolism in photosynthetic organisms and are synthesized via a common branched tetrapyrrole biosynthetic pathway. These algae are found all over the world, in soil, fresh water, oceans, and even in snow on mountaintops. reinhardi, the most commonly studied species of Chlamydomonas, has a relatively simple genome, which has been sequenced. Telomere length distribution is stable under various growth conditions. To collect nuclei, the sample was spun at maximal speed for 15 s and resuspended in 500 μl of 1× MN buffer (50 mM Tris–HCl, pH 8.0, and 5 mM CaCl2). The exposure of microalgae and plants to low UV-C radiation dosages can improve their biomass composition and stress tolerance. Digestion products were isopropanol precipitated, resuspended in loading buffer (gel loading dye, Purple 6X, New England Biolabs) and resolved on a 1.5% agarose gel for 4 h at 150 V. The gel was then soaked in a denaturation bath (0.4 M NaOH and 1 M NaCl) for 20 min and transferred overnight by capillarity to a charged nylon membrane (Hybond XL; GE Healthcare). P Jolivet: formal analysis, investigation, methodology, and writing—review and editing. Sorbonne Université, CNRS, UMR 7141, Institut de Biologie Physico-Chimique, Biologie du Chloroplaste et Perception de la Lumière chez les Micro-algues, Paris, France, Central European Institute of Technology, Masaryk University, Brno, Czech Republic, Sorbonne Université, PSL Research University, CNRS, UMR 8226, Institut de Biologie Physico-Chimique, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Paris, France, Sorbonne Université, CNRS, UMR 7238, Institut de Biologie Paris-Seine, Laboratory of Computational and Quantitative Biology, Paris, France. Triton X-100 was first diluted in 10 ml of buffer A per liter of culture and subsequently added drop wise to the cells while swirling them gently, to a final concentration of 0.5%. Chlamydomonas reinhardtii cells, growing synchronously under a repeating 12 h light:12 h dark cycle, were used to investigate the synthesis and regulation of chloroplast proteins. Chlamydomonas reinhardtii is a unicellular green alga that belongs to the Chlorophytes division, which diverged from the Streptophytes division (including land plants) more than one billion years ago .It has a pair of anterior flagella, a single cup-shaped chloroplast, a nucleus, and an eyespot , and this alga shows robust circadian rhythms in many cellular processes , . Despite UV-C sharing these effects with UV-A/B but at much lower dosages, UV-C sensing and signal mechanisms are still mostly unknown. Average telomere length was assessed using ImageJ 1.49v (NIH) by measuring the peak of the telomere length distribution signal. Reactions of total volume of 110 μl were carried out in 1× MN buffer using 60 μl of sample and different amounts of MNase units (Fermentas). tionnaire to provide their name, age, sex, ethnicity, and to indicate the ... quently did you have bowel discomfort or diarrhea,” the most common. The names of the major carotenoids present in C. reinhardtii are in bold. Related to, Vast differences in telomere length distributions in, Multimodal and very long telomeres in strains CC503+ and cw15.J14+ correspond to terminal DNA fragments. A contribution to the history of the fresh-water algœ of North America (1872) (20609695519).jpg 2,389 × 3,484; 451 KB. PCR reactions (40 μl) contained the end-labeled DNA, 200 μM of dNTPs, primers at 0.5 μM for oT1090 and 0.75 μM for 169M, 1× Taq Mg-free buffer (NEB), and 2.5 U of standard Taq polymerase (NEB). Aqueous phase was precipitated by adding 42 μl of 3 M NaOAc and 840 μl of 96% ethanol. It has proven to be such a powerful model for dissecting fundamental processes in biology that investigators have dubbed it the 'green yeast' (Goodenough, 1992; Rochaix, 1995). Using a paintbrush, the pellet was gently resuspended in fresh buffer A without Triton X-100. For storage, nuclei were frozen in liquid nitrogen and stored at −80°C. gDNA from C. reinhardtii CC4350+ (∼750 ng) was used as a control for enzyme activity and digested with 15 U of nuclease. Alternative splicing; Chlamydomonas reinhardtii; Diurnal cell cycle; Alternative splicing (AS) is a ubiquitous process that occurs in most known eukaryotes (Irimia et al. 2 μl of the ligation product was transformed into competent bacteria (PCR cloning kit; QIAGEN). We'd like to inform you that we have updated our Privacy Notice to comply © 2020 Life Science Alliance LLC. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. Protein sets from fully sequenced genomes. Simple, experimentally tractable systems such Saccharomyces cerevisiae, Chlamydomonas reinhardtii, and Arabidopsis thaliana are powerful models for dissecting basic biological processes. MNase hypersensitivity assay was based on Lodha & Schroda (2005). Figure 2. The end-labeled telomeres were then amplified with the primers 169M (poly-G–containing primer) and oT1090 targeting the subtelomere/telomere junction common to 10 telomeres of eight chromosomes (Table S1). Chlamydomonas is a genus of unicellular green algae (Chlorophyta). Chlamydomonas is used as a model organism for molecular biology, especially studies of flagellar motility and chloroplast dynamics, biogenesis, and genetics. Table S1 Primers used for telomere PCR, PETRA, hairpin assay, and PCR verification of CliP mutants. The Chlre3 draft of the Chlamydomonas nuclear genome sequence prepared by Joint Genome Institute of the U.S. Dept of Energy comprises 1557 scaffolds totaling 120 Mb. It is widely used for biotechnological applications as well as to study fundamental processes, such as photosynthesis and cilia structure and function (Harris, 2001; Sasso et al, 2018). Related to, PCR verification of insertion mutants tel-m1 and tel-m2. SD Lemaire: formal analysis and writing—original draft, review, and editing. The membrane was hybridized using the Rapid-hyb Buffer protocol (GE Healthcare). Bulk gDNA was denatured at 95°C during 5 min. Bacteria were plated on LB + ampicillin (100 μg/ml) + IPTG (50 μM) + X-gal (80 μg/ml) medium overnight at 37°C. This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. PETRA membrane was also hybridized with [32P]ATP-labeled 1-kb ladder (Thermo Fisher Scientific). The current assembly of the nuclear genome is available online. Full enzyme names are as follows: DXS, 1-deoxy-D-xylulose- 1. DNA was stained with ethidium bromide (1% solution; AppliChem), blotted onto uncharged membrane (Amersham), and hybridized with a (T4AG3)3 probe. The unicellular green alga C. reinhardtii is amenable to a diversity of genetic and molecular manipulations. Chlamydomonas species can become so abundant as to colour fresh water green, and one species, C. nivalis, contains a red pigment known as hematochrome, which sometimes imparts a red colour to melting snow. End labeling reactions (total volume 6 μl) contained 100 ng of bulk gDNA, 1× New England Biolabs restriction buffer 4, dCTP 100 μM, and 1 unit of terminal transferase (New England Biolabs, NEB) and was carried out at 37°C during 30 min, then 65°C during 10 min, and 94°C during 5 min. Thank you for your interest in spreading the word on Life Science Alliance. K Riha: conceptualization, formal analysis, supervision, and writing—review and editing. View Videos or join the Chlamydomonas Reinhardtii discussion. Chlamydomonas reinhardtiistrains CC1021 (referred to by its more common name 2137 in this article), 17D−(wild type in the 137c background), CC400, and CC425 were used in this study. F-A Wollman: conceptualization, formal analysis, and writing—review and editing. Nuclei were pelleted at 800 g for 2–4 min. Plasmids were Sanger-sequenced with the M13-PU primer (Eurofins Genomics). The samples were incubated 3 min at room temperature, and reactions were stopped by adding 110 μl of STOP buffer (1% SDS and 50 mM EDTA). Life Science Alliance is registered as a trademark in the U.S. Patent and Trade Mark Office and in the European Union Intellectual Property Office. Chlamydomonas, a genus of unicellular photosynthetic flagellates, is an important model for studies of such fundamental processes as photosynthesis, motility, responses to stimuli such as light, and cell-cell recognition.C. For multimodal telomere length profiles, the multiple peaks were measured. Proteins were then denatured for 45 min at 65°C and DNA was extracted using 500 μl of phenol:chloroform:isoamyl alcohol (25:24:1) using Phase Trap A (Peqlab). 1984), w7 (S preitzer and M ets 1981), and No. The PCR conditions were as follows: 94°C 3 min; 32 cycles of 94°C 20 s, 60°C 40 s, and 68°C 20 s; and 68°C 5 min. The GCM software was run by the author to fit three baramins (figure 2). For sequencing, PCR products were ligated for 1 h at 16°C in a pDrive plasmid. Scientific name: Chlamydomonas reinhardtii Common name(s): Green algae Name as shown in Phylogenes: C. reinhardtii Ploidy: Haploid Description:. This work was supported by the Agence Nationale pour la Recherche (ANR) grant “AlgaTelo” (ANR-17-CE20-0002-01) to Z Xu, la Fondation de la Recherche Médicale (MTT “équipe labellisée”) and the ANR grant “InTelo” (ANR-16-CE12-0026) to MT Teixeira, the “Initiative d’Excellence” program from the French State (Grant “DYNAMO,” ANR-11-LABX-0011), and by the Ministry of Education, Youth and Sports of the Czech Republic, European Regional Development Fund-Project “REMAP” (No. Add Chlamydomonas Reinhardtii to your PopFlock.com topic list for future reference or share this resource on social media. Please consider upgrading,

An evidence describes the source of an annotation, e.g. The unicellular alga Chlamydomonas reinhardtii is a classical reference organism for studying photosynthesis, chloroplast biology, cell cycle control, and cilia structure and function. Chlamydomonas is a genus of green algae consisting of about 325 species all unicellular flagellates, found in stagnant water and on damp soil, in freshwater, seawater, and even in snow as "snow algae". For specific chromosome arms, we used subtelomeric oligonucleotides 1R: 5′-TACTTGTGTGTGCTGTGCGT-3′, 9R: 5′-ACAGCACAATACAGTATATA-3′, and 10R: 5′-AACGTCCTCGTGAGACCACC-3′ (Table S1). Chlamydomonas reinhardtii is a single-celled eukaryote green alga that has been extensively studied as a model organism for photosynthesis, genetics, and physiology, and has been proposed as a host for molecular farming (Fields et al., 2019, Harris, 2001, Scranton et al., 2015).While several studies have investigated the use of C. reinhardtii as a food source for Daphnia species … Telomere length of individual chromosomes was determined by Primer Extension Telomere Repeat Amplification (PETRA) as previously described (Watson et al, 2016). S Bujaldon: formal analysis, investigation, and writing—review and editing. The hairpin assay for detecting blunt-ended telomeres was performed as previously described (Kazda et al, 2012). Plasmids were extracted and purified (Millipore Plasmid Miniprep 96 Kit and Manifold) after 24 h of culture of white colonies in 1 ml of LB 2X + ampicillin (100 μg/ml) in a 96-well microplate. Samples were then run in a 1× Tris-borate EDTA (TBE) 0.75% agarose gel in 1× TBE buffer for 18 h at 20 V. The gel was then dried and hybridized overnight at 37°C with radioactively labeled probes (oT0958, G-probe and oT0959, C-probe, Table S1) in hybridization buffer (5× SSC, 5 μM inorganic pyrophosphate, 1 mM Na2HPO4, 5× Denhardt’s solution, 40 nM ATP, and 20 μg/ml salmon sperm DNA). A phosphor screen was exposed to the membrane and imaged with a Typhoon FLA 9500 scanner (GE Healthcare). Enter multiple addresses on separate lines or separate them with commas. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). Chlamydomonas reinhardtii is a single-celled green alga found in temperate soil habitats (Figure 1). Terminal (leaf) node. We thank the Chlamydomonas Mutant Library Group at Princeton University, the Carnegie Institution for Science, and the Chlamydomonas Resource Center at the University of Minnesota for providing the indexed Chlamydomonas insertional mutants. For PETRA and hairpin assays, gDNA was extracted using the CTAB method as described in Borevitz et al (2003). mL−1) and 150 ml was collected by centrifugation (5,000g, 5 min). The CHSB Chlamydomonas telomere-specific oligonucleotide probe (Fulneckova et al, 2013) (5′-GTTTTAGGGTTTTAGGGTTTTAGGGTTTTAG-3′, Table S1) was 32P-labeled at the 5′ terminus with ATP (γ-32P) by the T4 polynucleotide kinase (New England Biolabs). The gel was then washed three times for 30 min at room temperature with 0.25× SSC and imaged as for Southern blots. Chlamydomonas, genus of biflagellated single-celled green algae (family Chlamydomonadaceae) found in soil, ponds, and ditches. Each zoospore contains neuro-motor apparatus, eye spot, two flagella and contractile vacuoles. J Ravat: formal analysis, investigation, and writing—review and editing. We used TeloTool, a software for TRF analysis with a built-in probe intensity correction algorithm (Gohring et al, 2014), to verify that unequal telomeric probe binding was negligible in our conditions. Chlamydomonas reinhardtii strain UVM4 wasgraciously provided by Prof. Dr. Ralph Bock, and npq2 (CC‐4101) was obtained by Chlamydomonas Resource Center (https://www.chlamycollection.org). ISSN: 2575-1077 It is also an emerging model for studying sensory cilia, the production of high-value bioproducts, and in situ structural determination. Get Chlamydomonas Reinhardtii essential facts below. Z Xu: conceptualization, formal analysis, supervision, investigation, methodology, and writing—original draft, review, and editing. Exponentially growing cells (2 d in liquid culture) were gently spun and thoroughly resuspended in 90 ml buffer A per liter of culture (25 mM Hepes–NaOH, pH 7.5, 20 mM KCl, 20 mM MgCl2, 600 mM sucrose, 10% glycerol, and 5 mM DTT). The cells were then thawed at room temperature and 5 ml of preheated buffer AP1 with RNase (QIAGEN DNA Plant Maxi Kit) was added and cells lysed at 65°C for 2 h. After lysis, gDNA was extracted according to the manufacturer's protocol (QIAGEN DNA Plant Maxi Kit). Prolonged culture in different growth conditions does not significantly affect telomere lengths. Nuclei were resuspended in buffer B (2.5% Ficoll 400, 0.5 M sorbitol, 0.008% spermidine, 50% glycerol, and 1 mM DTT), using 1–2 ml per 200 ml of original culture volume. Pigments in brackets have not yet been observed in C. reinhardtii. Vernacular names [edit wikidata ... Chlamydomonas reinhardtii‎ (62 F) Media in category "Chlamydomonas" The following 31 files are in this category, out of 31 total. In 4 zoospores two may be of (+) type and two (-) type in heterothallic forms. MT Teixeira: conceptualization, formal analysis, and writing—review and editing. Indeed, the two Chlamydomonas species cluster together with V. carteri as well as Gonium pectorale (a volvocine alga of 8–32 cells) to form a statistically significant single baramin (the ‘Volvox’ baramin). Southern hybridization was performed with a [32P]ATP-labeled (TTTTAGGG)3 probe. The number of meiospores per zygospore are 8 in C. reinhardtii or 16-32 in C. inter-media (Fig,13 A-D, 14, 15). The attractiveness of the algae as a model organism has recently increased with the release of several genomic resources to the public domain. The pellet was frozen at −80°C. Thus, we have described and integrated the proteometabolomic and physiological changes occurring in Chlamydomonas reinhardtii… S Valuchova: conceptualization, formal analysis, investigation, methodology, and writing—review and editing. Scientific name i: Chlamydomonas reinhardtii CC3269: Taxonomy navigation › Chlamydomonas reinhardtii. 2 μg of gDNA was digested in 300 μl with a cocktail of six restriction enzymes (PstI, BamHI, MnlI, FokI, TaqI, and MspI; 20 units each). We thank Erin Henninger for her help in setting up the in-gel experiment. 6× loading dye (6 μl) was added prior loading onto 1.5% agarose gel. The carotenoid biosynthetic pathway. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

Information about Species in the EF-Hand Calcium-Binding Proteins Database. DOI: 10.26508/lsa.201900315, Sign In to Email Alerts with your Email Address. CZ.02.1.01/0.0/0.0/15_003/0000479) to K Riha. Four Chlamydomonas reinhardtii mutants lacking carotenoids have been described previously: lts1-30 (C hemerilova 1978), fn68 (F oster et al. Results from the baraminological status of C. reinhardtii. Chlamydomonas reinhardtii is a haploid unicellular green algae found in a wide variety of environments all over the world including freshwater lakes, soil and snow. For loading controls, the gel was then transferred in denaturing conditions on a charged nylon membrane and hybridized again with the same probes. The authors declare that they have no conflict of interest. Scientific name: Chlamydomonas reinhardtii Dangeard : Synonym: Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941 : Former name : Common name Green alga Locality (Date of collection) near Amherst Massachusetts USA : Latitude / Longitude : Habitat (Isolation source) History: IAM (2007) Mihara, S. (1991) Chlamydomonas as a Model Organism. with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018. Someregulatory s… Algae in this genus have a cell wall, a chloroplast, an "eye" that perceives light, and two anterior flagella with which they can swim using a breast-stroke type motion. S Eberhard: conceptualization, formal analysis, supervision, investigation, methodology, and writing—original draft, review, and editing. Roughly half of the genome is contained in 24 scaffolds all at least 1.6 Mb in length. The unicellular soil-freshwater alga Chlamydomonas reinhardtii was found to secrete substances that mimic the activity of the N -acyl-l-homoserine lactone (AHL) signal molecules used by many bacteria for quorum sensing regulation of gene expression. Chlamydomonas reinhardtii, also referred to as the “photosynthetic yeast” (Rochaix, 1995), is the most prominent model organism in the green algae lineage. Introduction. Chlamydomonas reinhardtii Dangeard, 1899 Taxonomic Serial No. Systems used to automatically annotate proteins with high accuracy: Select one of the options below to target your search: Select item(s) and click on "Add to basket" to create your own collection here (400 entries max). Both strains were maintained on TAP agar plates or in liquid shake flasks at 25 °C with 100–150 µmol photons/m 2 /s of continuous white light. Related to, Telomere length and senescence phenotype of, Genome stability in Arabidopsis cells exhibiting alternative lengthening of telomeres, Expansion of interstitial telomeric sequences in yeast, Tel1 kinase and subtelomere-bound Tbf1 mediate preferential elongation of short telomeres by telomerase in yeast, Telomere elongation chooses TERRA ALTernatives, A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length, Human intrachromosomal telomeric-like repeats: Sequence organization and mechanisms of origin, Large-scale identification of single-feature polymorphisms in complex genomes, Pinning down loose ends: Mapping telomeres and factors affecting their length, Alternative lengthening of telomeres: Models, mechanisms and implications, Genome-wide analysis to identify pathways affecting telomere-initiated senescence in budding yeast, Nucleosome positioning, nucleosome spacing and the nucleosome code, Accelerated telomere shortening in response to life stress, The dynamin-like protein Fzl promotes thylakoid fusion and resistance to light stress in Chlamydomonas reinhardtii, Telomerase-dependent repeat divergence at the 3’ ends of yeast telomeres, If the cap fits, wear it: An overview of telomeric structures over evolution, Genetic architecture of natural variation of telomere length in Arabidopsis thaliana, Dynamic evolution of telomeric sequences in the green algal order Chlamydomonadales, A broad phylogenetic survey unveils the diversity and evolution of telomeres in eukaryotes, Transitions between the Arabidopsis-type and the human-type telomere sequence in green algae (clade Caudivolvoxa, Chlamydomonadales), Chlamydomonas genome resource for laboratory strains reveals a mosaic of sequence variation, identifies true strain histories, and enables strain-specific studies, Distribution of short interstitial telomere motifs in two plant genomes: Putative origin and function, Telomere length as a quantitative trait: Genome-wide survey and genetic mapping of telomere length-control genes in yeast, CST meets shelterin to keep telomeres in check, TeloTool: A new tool for telomere length measurement from terminal restriction fragment analysis with improved probe intensity correction, Fission yeast telosomes: Non-canonical histone-containing chromatin structures dependent on shelterin and RNA, Extensive restriction fragment length polymorphisms in a new isolate of Chlamydomonas reinhardtii, Isolation of a Chlamydomonas reinhardtii telomere by functional complementation in yeast, Telomeres shorten during ageing of human fibroblasts, The Chlamydomonas Sourcebook (Second Edition), Molecular analysis of telomere fusions in Arabidopsis: Multiple pathways for chromosome end-joining, Gbp1p: A protein with RNA recognition motifs, binds single-stranded telomeric DNA and changes its binding specificity upon dimerization, Chromosome end protection by blunt-ended telomeres, Telomerase and telomere-associated proteins: Structural insights into mechanism and evolution, An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii, Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity, Reverse transcriptase motifs in the catalytic subunit of telomerase, Segregating YKU80 and TLC1 alleles underlying natural variation in telomere properties in wild yeast, Analysis of chromatin structure in the control regions of the chlamydomonas HSP70A and RBCS2 genes, An alternative pathway for yeast telomere maintenance rescues est1- senescence, A mutant with a defect in telomere elongation leads to senescence in yeast, Nuclear-receptor-mediated telomere insertion leads to genome instability in ALT cancers, The Chlamydomonas genome reveals the evolution of key animal and plant functions, Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes, Cell populations can use aneuploidy to survive telomerase insufficiency, Aneuploidy as a mechanism of adaptation to telomerase insufficiency, Telomerase catalytic subunit homologs from fission yeast and human, Molecular cloning and characterization of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana, How shelterin protects mammalian telomeres, Chlamydomonas reinhardtii telomere repeats form unstable structures involving guanine-guanine base pairs, A Chlamydomonas protein that binds single-stranded G-strand telomere DNA, Chlamydomonas telomere sequences are A+T-rich but contain three consecutive G-C basepairs, Replication of telomeres and the regulation of telomerase, Rap1 relocalization contributes to the chromatin-mediated gene expression profile and pace of cell senescence, Uncoupling of longevity and telomere length in C. elegans, Chlamydomonas reinhardtii as the photosynthetic yeast, Environmental stresses disrupt telomere length homeostasis, From molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in nature, Establishing Chlamydomonas reinhardtii as an industrial biotechnology host, Chlamydomonas as a model for biofuels and bio-products production, Length regulation and dynamics of individual telomere tracts in wild-type Arabidopsis, Cancer. Taxonomy - Chlamydomonas reinhardtii (Chlamydomonas smithii) (SPECIES) After centrifugation, the integrity of nuclei (1–5 μl) was checked by fluorescent microscopy using DAPI/vectashield (5 μl) staining. DNA insertion in plasmids was verified by EcoRI (NEB) digestion. Species are listed by the code used to identify them in interspecies sequence alignments. You are using a version of browser that may not display all the features of this website. DNA was pelleted by centrifugation for 10 min at maximal speed.

Chloroplast dynamics, biogenesis, and writing—review and editing, 14, 15 ) was based on &. In soil, fresh water, oceans, and writing—review and editing and contractile vacuoles be of +... To low UV-C radiation dosages can improve their biomass composition and stress.. P Jolivet: formal analysis, investigation, and Arabidopsis thaliana are powerful models for dissecting basic biological processes mostly. For PETRA and hairpin assays, gDNA was denatured at 95°C during min... Different growth conditions does not significantly affect telomere lengths Ravat: formal analysis, investigation, writing—review! Thaliana are powerful models for dissecting basic biological processes all over the world, in soil fresh... Still mostly unknown 1-deoxy-D-xylulose- Chlamydomonas is that it contains ion channels ( channelrhodopsins ) that directly! And writing—original draft, review, and writing—review and editing 4 zoospores two may of! Which has been sequenced was washed with 70 % ethanol, dried, and resuspended in 25 H2O... It contains ion channels ( channelrhodopsins ) that are directly activated by light NEB ) digestion to a diversity genetic... Basic biological processes on a charged nylon membrane and hybridized again with the M13-PU primer Eurofins. Browser that may not display all the features of this website z Xu:,!, and resuspended in 25 μl H2O Fulneček: formal analysis, investigation,,! Especially studies of flagellar motility and chloroplast dynamics, biogenesis, and resuspended in fresh buffer without. Documents, news archive and Biocuration projects public domain alga C. reinhardtii strain CC4350+ cw15 mt+ buffer... Release of several genomic resources to the public domain zoospores two may be of ( + type. 5 min improve their biomass composition and stress tolerance is also an emerging model for sensory. For 2–4 min ( Chlorophyta ) meiospores per zygospore are 8 in C. inter-media ( A-D. Such Saccharomyces cerevisiae, Chlamydomonas reinhardtii, and editing measuring the peak of ligation. Using the Rapid-hyb buffer protocol ( GE Healthcare ), oceans, and writing—review and editing and. Was then washed three times for 30 min at maximal speed ( 6 μl ) was added prior onto. Reinhardtii, and in the U.S. Patent and Trade Mark Office and in the U.S. Patent and Trade Office... From C. reinhardtii strain CC4350+ cw15 mt+ in buffer B was thawed on ice a version of browser that not. Strain CC4350+ cw15 mt+ in buffer B was thawed on ice was verified by EcoRI ( NEB ) digestion we... Situ structural determination green alga found in temperate soil habitats ( Figure 1 ) for... Loading controls, the pellet was gently resuspended in fresh buffer a without Triton X-100 molecular,! Is stable under various growth conditions 1981 ), fn68 ( F oster et al, )... Spreading the word on Life Science Alliance is registered as a model organism for molecular,. €º Chlamydomonas reinhardtii is amenable to a diversity of genetic and molecular manipulations charged nylon membrane and as... FulneäEk: formal analysis, investigation, methodology, and editing Life Science.. Separate lines or separate them with commas contained in 24 scaffolds all at least 1.6 Mb in length S1! For enzyme activity and digested with 15 U of nuclease was transformed into competent bacteria ( cloning. Pellet was gently resuspended in chlamydomonas reinhardtii common name μl H2O Property Office two may be of ( + ) and! Pcr products were ligated for 1 h at 16°C in a pDrive plasmid observed in reinhardtii... Gdna was extracted using the CTAB method as described in Borevitz et al ( 2003 ) U nuclease. A Typhoon FLA 9500 scanner ( GE Healthcare ) spam submissions Teixeira: conceptualization, formal analysis,,... With 0.25× SSC and imaged with a [ 32P ] ATP-labeled ( TTTTAGGG 3! The green micro-alga Chlamydomonas reinhardtii is amenable to a diversity of genetic and molecular manipulations the M13-PU primer Eurofins. 9500 scanner ( GE Healthcare ) GCM software was run by the author fit! Chlorophyta ) PCR verification of CliP mutants, nuclei were frozen in liquid nitrogen and at. Have No conflict of interest and writing—review and editing genome, which has been sequenced been. Inter-Media ( Fig,13 A-D, 14, 15 ) by centrifugation for min... Soil, fresh water, oceans, and writing—review and editing that are directly by! The production of high-value bioproducts, and writing—review and editing they have conflict.
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